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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has actually been noticed in lots of tumors, which leads to chromosome instability, and therefore may contribute to tumorigenesis. This protein was observed to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a essential job in advertising and marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been described. Two additional splice variants were being reported but in depth nucleotide sequence information and facts will not be nonetheless accessible.

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Blocking peptides are peptides that bind particularly on the target antibody and block antibody binding. These peptide typically incorporates the epitope recognized with the antibody.

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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to make certain the absolute best success are...

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This guideline outlines additional doable optimization of mobile and immunoassay parameters to be sure the best possible outcomes are...

Antibodies bound to the blocking peptide not bind to your epitope about the concentrate on protein. By evaluating the staining within the blocked antibody compared to the antibody on your own, you can see which staining is particular.

Concentrate on Info Cyclin E belongs towards the extremely conserved cyclin household, whose users are characterised by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins perform as regulators of CDK kinases. Unique cyclins show distinct expression and degradation designs which add towards the temporal coordination of every mitotic occasion. Cyclin E sorts a complex with and features as being a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress through S phase.

The measurement of protein phosphorylation is a useful gizmo website for measuring the modulation of receptor activation by the two antibodies...

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